ISO 21149:2006 pdf download.Cosmetics - Microbiology - Enumeration and detection of aerobic mesophilic bacteria.
8 Handling of cosmetic products and laboratory samples
If necessary store products to be tested at room temperature. Do not Incubate, refrigerate or freeze products (3.2) and samples (3.3) before or after analysis.
Sampling of cosmetic products to be analysed should be carried out, as described in ISO 21148. Analyse samples as specified in ISO 21148 and in accordance with the fufluwiny procedure
9 Procedure
9.1 General recommendation
Use sterile material, equipment and aseptic techniques to prepare the sample, initial suspension and dilutions. In the case of the preparation of an initial suspension, the time which elapses between the end of the preparation and the moment the inoculum comes into contact with the culture medium shall not exceed 45 mm, unless specificaMy mentioned in the established protocols or documents.
9.2 PreparatIon of the Initial suspension
9.2.1 General
The initial suspension is prepared from a sample (3.3) of at least 1 g or 1 ml of the well-mixed product (3.2) under test
Note S the exact mass or volume of the sample.
The initial suspension is usually 1:10 diluten. Larger volumes of diluent or enrichment broth may be required If high levels of contamination are expected andlor if anti-microbial properties are still present in 1:10 dilution.
If necessary store products to be tested at room temperature. Do not Incubate, refrigerate or freeze products (3.2) and samples (3.3) before or after analysis.
Sampling of cosmetic products to be analysed should be carried out, as described in ISO 21148. Analyse samples as specified in ISO 21148 and in accordance with the fufluwiny procedure
9 Procedure
9.1 General recommendation
Use sterile material, equipment and aseptic techniques to prepare the sample, initial suspension and dilutions. In the case of the preparation of an initial suspension, the time which elapses between the end of the preparation and the moment the inoculum comes into contact with the culture medium shall not exceed 45 mm, unless specificaMy mentioned in the established protocols or documents.
9.2 PreparatIon of the Initial suspension
9.2.1 General
The initial suspension is prepared from a sample (3.3) of at least 1 g or 1 ml of the well-mixed product (3.2) under test
Note S the exact mass or volume of the sample.
The initial suspension is usually 1:10 diluten. Larger volumes of diluent or enrichment broth may be required If high levels of contamination are expected andlor if anti-microbial properties are still present in 1:10 dilution.
